腔肠素 Coelenterazine (ultra pure)
Cell viability dye
Colenterazine is useful for monitoring calcium in live cells and tissues, in reporter gene assays, for superoxide anion detection and in drug high-throughput screening applications. Coelenterazine is useful for reconstituting aequorin in cells that have been transfected with apo-aequorin cDNA. The aequorin complex comprises a 22kDa apoaequorin protein, molecular oxygen and coelenterazine. In the presence of Ca(II), coelenterazine is oxidized to coelenteramide, yielding the release of carbon dioxide and blue light.
PRODUCT PROPERTIES
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Alternative Name: |
2-[(4-Hydroxyphenyl)methyl]-6-(4-hydroxyphenyl)-8-(phenylmethyl)-imidazo [1,2-a] pyrazin-3-(7H)-one |
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Solubility: |
Soluble in 100% ethanol. |
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Long Term Storage: |
-20°C |
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Use/Stability: |
Store at -20°C, under nitrogen. Desiccation recommended. Protect material from long-term exposure to light; may be exposed to light for short periods of time. This material is unstable in DMSO, DMF, dimethylacetamide and N-methylpyrrolidone. Coelenterazine solution in methanol showed <10% decomposition when stored at room temperature in the dark for one month, and <2% decomposition when stored at –20°C in the dark, under nitrogen. |
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Handling: |
Keep cool and dry. |
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MW: |
423.5 |
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CAS: |
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Purity: |
≥90% (HPLC) |
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